Identifcation of Key Genes and Pathways Related to Nonalcoholic Fatty Liver Disease based on GEO Databases

Zheng Liang; July Liang Chen

doi:10.54691/3716ej02

Identifcation of Key Genes and Pathways Related to Nonalcoholic Fatty Liver Disease based on GEO Databases

Zheng Liang, July Liang Chen

Frontiers in Science and Engineering, (2024), Vol.4, No.4, pp.174-180

Published: April 30, 2024

DOI: 10.54691/3716ej02

PDF: Download Full Text PDF

Abstract

Background: Non-alcoholic fatty liver disease (NAFLD) represents a growing global health crisis and is closely associated with increases in obesity and type 2 diabetes. Despite its ubiquity, its underlying biochemical mechanisms and effective therapeutic strategies remain poorly defined, hampering the development of targeted interventions. Methods: Candidate genes were obtained from the GEO database, and Kyoto Encyclopedia of Genes and Genomes enrichment analysis and gene set enrichment analysis were used to identify pathways involved in NAFLD-related pathways. The top genes with higher degree in the protein-protein interaction network were crossed with the top genes enriched in key pathways, and then the correlation analysis between key genes and chemotherapy response was performed. Result: fatty acid metabolic process, lipid transport, response to stilbenoid, etc. are key pathways related to NAFLD. Btc, Apoa4, Gdf6 AND Ifi2712b were enriched in key pathways of NAFLD. Conclusion: Fatty acid metabolic process, lipid transport, pathway are key pathways related to NAFLD.

Keywords: NAFLD; GEO; KEGG.

APA Citation: Zheng Liang, July Liang Chen (2024). Identifcation of Key Genes and Pathways Related to Nonalcoholic Fatty Liver Disease based on GEO Databases. Frontiers in Science and Engineering, 4(4), 174-180. https://doi.org/10.54691/3716ej02

References

C-P Day, James O-F. Steatohepatitis: a tale of two "hits"?[J]. Gastroenterology, 1998, 114(4): 842-845.
R Loomba, Friedman S-L, Shulman G-I. Mechanisms and disease consequences of nonalcoholic fatty liver disease[J]. Cell, 2021, 184(10): 2537-2564.
H Tilg, Moschen A-R. Evolution of inflammation in nonalcoholic fatty liver disease: the multiple parallel hits hypothesis[J]. Hepatology, 2010, 52(5): 1836-1846.
M Mota, Banini B-A, Cazanave S-C, et al. Molecular mechanisms of lipotoxicity and glucotoxicity in nonalcoholic fatty liver disease[J]. Metabolism, 2016, 65(8): 1049-1061.
Q Ren, Sun Q, Fu J. Dysfunction of autophagy in high-fat diet-induced non-alcoholic fatty liver disease[J]. Autophagy, 2024, 20(2): 221-241.
U-S Udoh, Rajan P-K, Nakafuku Y, et al. Cell Autophagy in NASH and NASH-Related Hepatocellular Carcinoma[J]. Int J Mol Sci, 2022, 23(14).
Y Filali-Mouncef, Hunter C, Roccio F, et al. The menage a trois of autophagy, lipid droplets and liver disease[J]. Autophagy, 2022, 18(1): 50-72.
N Martinez-Lopez, Singh R. Autophagy and Lipid Droplets in the Liver[J]. Annu Rev Nutr, 2015, 35215-237.
H-S Park, Song J-W, Park J-H, et al. TXNIP/VDUP1 attenuates steatohepatitis via autophagy and fatty acid oxidation[J]. Autophagy, 2021, 17(9): 2549-2564.
S Byun, Seok S, Kim Y-C, et al. Fasting-induced FGF21 signaling activates hepatic autophagy and lipid degradation via JMJD3 histone demethylase[J]. Nat Commun, 2020, 11(1): 807.
X Deng, Liu B, Jiang Q, et al. CREBH promotes autophagy to ameliorate NASH by regulating Coro1a[J]. Biochim Biophys Acta Mol Basis Dis, 2024, 1870(1): 166914.
E-M Trifylli, Kriebardis A-G, Koustas E, et al. The Emerging Role of Extracellular Vesicles and Autophagy Machinery in NASH-Future Horizons in NASH Management[J]. Int J Mol Sci, 2022, 23(20).
H Qian, Chao X, Williams J, et al. Autophagy in liver diseases: A review[J]. Mol Aspects Med, 2021, 82100973.
S Zhao, Guo Y, Yin X. Lipid Peroxidation in Ferroptosis and Association with Nonalcoholic Fatty Liver Disease[J]. Front Biosci (Landmark Ed), 2023, 28(12): 332.
Z Cheng, Chu H, Zhu Q, et al. Ferroptosis in non-alcoholic liver disease: Molecular mechanisms and therapeutic implications[J]. Front Nutr, 2023, 101090338.
L Zhu, Luo S, Zhu Y, et al. The Emerging Role of Ferroptosis in Various Chronic Liver Diseases: Opportunity or Challenge[J]. J Inflamm Res, 2023, 16381-389.
G Guo, Yang W, Sun C, et al. Dissecting the potential role of ferroptosis in liver diseases: an updated review[J]. Free Radic Res, 2023, 57(4): 282-293.
Y Gong, Liu Z, Zhang Y, et al. AGER1 deficiency-triggered ferroptosis drives fibrosis progression in nonalcoholic steatohepatitis with type 2 diabetes mellitus[J]. Cell Death Discov, 2023, 9(1): 178.
X Zhou, Fu Y, Liu W, et al. Ferroptosis in Chronic Liver Diseases: Opportunities and Challenges[J]. Front Mol Biosci, 2022, 9928321.
S Wei, Qiu T, Wang N, et al. Ferroptosis mediated by the interaction between Mfn2 and IREalpha promotes arsenic-induced nonalcoholic steatohepatitis[J]. Environ Res, 2020, 188109824.
W Xu, Hu Z, Zhang J, et al. Cross-talk between autophagy and ferroptosis contributes to the liver injury induced by fluoride via the mtROS-dependent pathway[J]. Ecotoxicol Environ Saf, 2023, 250114490.
B Zhou, Liu J, Kang R, et al. Ferroptosis is a type of autophagy-dependent cell death[J]. Semin Cancer Biol, 2020, 6689-100.
Z Zhang, Guo M, Li Y, et al. RNA-binding protein ZFP36/TTP protects against ferroptosis by regulating autophagy signaling pathway in hepatic stellate cells[J]. Autophagy, 2020, 16(8): 1482-1505.
J Liu, Huang C, Liu J, et al. Nrf2 and its dependent autophagy activation cooperatively counteract ferroptosis to alleviate acute liver injury[J]. Pharmacol Res, 2023, 187106563.